Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A
Last modified: 10/Sep/2021
Results
Publication
Ke PY, Kuo YY, Hu CM, Chang ZF: Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability., Genes Dev, 2005

PubMed PMCID
Value 129.77
Dimension pmol/106 cell
Error (standard deviation) 17.93
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
CLO Cell line name HeLa cell (CLO:0003684)
Cell line/type as in publication HeLa
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Sherman and Fyfe
Growth conditions cells were transfected with siRNA corresponding to Cdh1, then maintained in DMEM with 10% fetal bovine serum and antibiotics for 48 hours
Treatment
Treatment details/effects silencing of Fizzy-related protein homolog (Cdh1), substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex
Genes and Proteins
Gene name FZR1
Gene identifier 51343
Protein name Fizzy-related protein homolog
Protein identifier Q9UM11
Genetic manipulation/variation silencing