Results
Publication	Gramberg T, Kahle T, Bloch N, Wittmann S, Müllers E, Daddacha W, Hofmann H, Kim B, Lindemann D, Landau NR: Restriction of diverse retroviruses by SAMHD1., Retrovirology, 2013 PubMed PMCID
Value	0.1955
Dimension	pmol/106 cell
Error	Error not provided.
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
CLO Cell line name	THP-1 cell (CLO:0009348)
Cell line/type as in publication	THP-1 monocytic cell line
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Diamond et al. 2004., single-nucleotide extension assay
Growth conditions	cells were differentiated with phorbol myristic acid (PMA) for 20 h

Treatment
Treatment details/effects	silencing of SAMHD1 with shRNA and extrachromosomal expression of shRNA-resistant catalitically inactive SAMHD1, these together result in SAMHD1 with loss of function

Genes and Proteins
Gene name	SAMHD1
Gene identifier	25939
Protein name	SAMHD1
Protein identifier	Q9Y3Z3
Genetic manipulation/variation	extrachromosomal expression of mutant protein
Mutation in protein	shRNA-resistant SAMHD1 with H206A, D207A mutations