Results
Publication	Bloch N, Gläsker S, Sitaram P, Hofmann H, Shepard CN, Schultz ML, Kim B, Landau NR: A Highly Active Isoform of Lentivirus Restriction Factor SAMHD1 in Mouse., J Biol Chem, 2017 PubMed PMCID
Value	11.8
Dimension	pmol/mg protein
Error (unspecified)	2.6
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
CLO Cell line name	U-937 cell (CLO:0009465)
Cell line/type as in publication	U937 cells
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Diamond et al. 2004., single-nucleotide extension assay
Growth conditions	U937 cells were transduced with lentiviral vector encoding P635A mutated ISF1 (isoform1) of SAMHD1

Treatment
Treatment details/effects	perturbation of the dNTP pool by dNTP triphosphohydrolase

Genes and Proteins
Gene name	SAMHD1
Gene identifier	25939
Protein name	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein identifier	Q9Y3Z3
Genetic manipulation/variation	extrachromosomal expression of mutant protein
Mutation in protein	P635A, which disrupts the CDK consensus phosphorylation motif and prevents phosphorylation