2'-Deoxyadenosine 5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A
Last modified: 10/Sep/2021
Results
Publication
Bloch N, Gläsker S, Sitaram P, Hofmann H, Shepard CN, Schultz ML, Kim B, Landau NR: A Highly Active Isoform of Lentivirus Restriction Factor SAMHD1 in Mouse., J Biol Chem, 2017

PubMed PMCID
Value 7.9
Dimension pmol/mg protein
Error (unspecified) 2.6
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
CLO Cell line name U-937 cell (CLO:0009465)
Cell line/type as in publication U937 cells
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Diamond et al. 2004., single-nucleotide extension assay
Growth conditions U937 cells were transduced with lentiviral vector encoding ISF2 (isoform2) of SAMHD1
Treatment
Treatment details/effects perturbation of the dNTP pool by dNTP triphosphohydrolase
Genes and Proteins
Gene name SAMHD1
Gene identifier 25939
Protein name Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein identifier Q9Y3Z3
Genetic manipulation/variation extrachromosomal protein expression