DNTP004910
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001245 |
| Members of this Pool |
|
| Members of control pools |
|---|
| POOL001242 |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Koehler SE, Ladner RD: Small interfering RNA-mediated suppression of dUTPase sensitizes cancer cell lines to thymidylate synthase inhibition.,
Mol Pharmacol, 2004
PubMed |
| Value | 1 | Dimension | pmol/106 cell |
| Error (standard deviation) | 0.1 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | colon |
| CLO Cell line name | HT-29 cell (CLO:0004283) |
| Cell line/type as in publication | HT-29 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | acid extraction |
| Remarks to measurement type | Sherman and Fyfe |
| Growth conditions | 24 h with 1 μM FUdR |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 5790 |
| Applied drug | 5-Fluorodeoxyuridine |
| Treatment details/effects | perturbation of thymidylate synhtesis pathway by silencing dUTPase |
| Genes and Proteins | |
|---|---|
| Gene name | dut |
| Gene identifier | 1854 |
| Protein name | dUTPase |
| Protein identifier | P33316 |
| Genetic manipulation/variation | silencing |