Results
Publication	Wheeler LJ, Rajagopal I, Mathews CK: Stimulation of mutagenesis by proportional deoxyribonucleoside triphosphate accumulation in Escherichia coli., DNA Repair (Amst), 2005 PubMed
Value	21.9
Dimension	pmol/108 CFU
Error	Error not provided.
Data presentation in publication	numerical

Source
Source organism	E. coli (Escherichia coli) Taxonomy 562
Bacterial or fungal strain	Tuner/DE3/pet115b
Sample source	strain
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Mathews and Wheeler
Growth conditions	N/A

Treatment
Treatment details/effects	mutagenized nrdBgene (in plasmid) to change the catalytically essential tyrosine-122 to phenylalanine, a change known to abolish enzyme activity

Genes and Proteins
Gene name	nrda
Gene identifier	946612
Protein name	Ribonucleoside-diphosphate reductase 1 subunit alpha
Protein identifier	P00452
Genetic manipulation/variation	extrachromosomal protein expression
Gene name	nrdb
Gene identifier	946732
Protein name	Ribonucleoside-diphosphate reductase 1 subunit beta
Protein identifier	P69924
Genetic manipulation/variation	extrachromosomal expression of mutant protein
Mutation in protein	Y122F