2'-Deoxyadenosine 5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001146
Members of this Pool
Members of control pools
POOL001138
Last modified: 10/Sep/2021
Results
Publication
Das SK, Benditt EP, Loeb LA: Rapid changes in deoxynucleoside triphosphate pools in mammalian cells treated with mutagens., Biochem Biophys Res Commun, 1983

PubMed
Value 82.0448
Dimension pmol/106 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism Chinese hamster (Cricetulus griseus)
Taxonomy 10029
Sample source cell line
Tissue type fibroblasts
Cell line/type as in publication V79
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Skoog
Growth conditions Exponentially growing V79 cells treated with MNNG: MNNG at a final concentration of 5 μM was added at -1 hr: At the end of 1 hr incubation at 37 °C the medium was replaced with fresh medium without MNNG. 2 h after treatment.
Treatment
Drug/stress type drug + stress
PubChem 135436526
Applied drug 1-Methyl-3-Nitro-1-Nitrosoguanidine
Treatment details/effects mutagen; MNNG treatment caused V79 cells to be blocked in S phase
Genes and Proteins
No genes or proteins manipulated.