2'-Deoxyadenosine 5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001008
Members of this Pool
Last modified: 10/Sep/2021
Results
Publication
Purhonen J, Banerjee R, McDonald AE, Fellman V, Kallijärvi J: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase., Nucleic Acids Res, 2020

PubMed PMCID
Value 8.2
Dimension pmol/106 cell
Error (standard deviation) 0.9
Data presentation in publication numerical
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
CLO Cell line name HCT 116 cell (CLO:0003665)
Cell line/type as in publication HCT116
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type method article; Q5 DNA polymerase and EvaGreen-based assay for dNTPs using 197-nt templates
Growth conditions N/A
Treatment
There was no treatment in this experiment.
Genes and Proteins
No genes or proteins manipulated.