Results
Publication	Purhonen J, Banerjee R, McDonald AE, Fellman V, Kallijärvi J: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase., Nucleic Acids Res, 2020 PubMed PMCID
Value	23
Dimension	pmol/106 cell
Error (standard deviation)	3.8
Data presentation in publication	numerical

Source
Source organism	mouse (Mus musculus) Taxonomy 10090
Sample source	cell line
CLO Cell line name	Hepa 1-6 cell (CLO:0003711)
Cell line/type as in publication	Hepa 1-6
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	method article; Q5 DNA polymerase and EvaGreen-based assay for dNTPs using 197-nt templates
Growth conditions	200 nM myxothiazol for 6h; vector with PoAOX gene

Treatment
Drug/stress type	drug + stress
PubChem	10972974
Applied drug	myxothiazol
Treatment details/effects	transfection; inhibition of pyrimidine biosynthesis by blocking mitochondrial respiratory complex III

Genes and Proteins
Gene name	AOX
Gene identifier	105322704
Protein name	alternative oxidase
Protein identifier	D2SP10
Genetic manipulation/variation	extrachromosomal protein expression