DNTP004010
dATP
dNTP Pool | |
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Pool identifier | POOL001006 |
Members of this Pool |
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Members of control pools |
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POOL001005 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Purhonen J, Banerjee R, McDonald AE, Fellman V, Kallijärvi J: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase.,
Nucleic Acids Res, 2020
PubMed PMCID |
Value | 4.6 | Dimension | pmol/106 cell |
Error (standard deviation) | 1.5 |
Data presentation in publication | numerical |
Source | |
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Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
Sample source | cell line |
CLO Cell line name | Hepa 1-6 cell (CLO:0003711) |
Cell line/type as in publication | Hepa 1-6 |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | method article; Q5 DNA polymerase and EvaGreen-based assay for dNTPs using 197-nt templates |
Growth conditions | 200 nM myxothiazol for 6h; empty vector |
Treatment | |
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Drug/stress type | drug + stress |
PubChem | 10972974 |
Applied drug | myxothiazol |
Treatment details/effects | transfection; inhibition of pyrimidine biosynthesis by blocking mitochondrial respiratory complex III |
Genes and Proteins | |
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No genes or proteins manipulated. | |