DNTP004003
dCTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001004 |
| Members of this Pool |
|
| Members of control pools |
|---|
| POOL001003 |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Purhonen J, Banerjee R, McDonald AE, Fellman V, Kallijärvi J: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase.,
Nucleic Acids Res, 2020
PubMed PMCID |
| Value | 67 | Dimension | pmol/106 cell |
| Error (standard deviation) | 7.2 |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
| Sample source | cell line |
| CLO Cell line name | Hepa 1-6 cell (CLO:0003711) |
| Cell line/type as in publication | Hepa 1-6 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | method article; Q5 DNA polymerase and EvaGreen-based assay for dNTPs using 197-nt templates |
| Growth conditions | 5 μM 5-fluorouracil for 6 h |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 3385 |
| Applied drug | 5-fluorouracil |
| Treatment details/effects | Thymidylate synthase inhibitor that blocks dTTP biosynthesis |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |