DNTP003994
dATP
| dNTP Pool | |
|---|---|
| This entry does not belong to any pool in the database. |
| Members of control pools |
|---|
| N/A |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Sommer AF, Rivière L, Qu B, Schott K, Riess M, Ni Y, Shepard C, Schnellbächer E, Finkernagel M, Himmelsbach K, Welzel K, Kettern N, Donnerhak C, Münk C, Flory E, Liese J, Kim B, Urban S, König R: Restrictive influence of SAMHD1 on Hepatitis B Virus life cycle.,
Sci Rep, 2016
PubMed PMCID |
| Value | 0.1521 | Dimension | pmol/106 cell |
| Error (standard error of the mean) | 0.0104 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| CLO Cell line name | Hep G2 cell (CLO:0003704) |
| Cell line/type as in publication | HepG2 cells |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | with HIV-1 reverse transcriptase |
| Growth conditions | transfected with 1 μg of plasmid coding for wild-type FLAG-tagged SAMHD1; 24 hours after transfection, the cells were washed and cultured in serum-free medium without supplements to arrest the cell cycle; quantification of dATP levels were 3 days post medium change |
| Treatment | |
|---|---|
| Drug/stress type | stress |
| Treatment details/effects | transfection |
| Genes and Proteins | |
|---|---|
| Gene name | SAMHD1 |
| Gene identifier | 56045 |
| Protein name | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 |
| Protein identifier | Q60710 |
| Genetic manipulation/variation | extrachromosomal protein expression |