Results
Publication	Thientosapol ES, Bosnjak D, Durack T, Stevanovski I, van Geldermalsen M, Holst J, Jahan Z, Shepard C, Weninger W, Kim B, Brink R, Jolly CJ: SAMHD1 enhances immunoglobulin hypermutation by promoting transversion mutation., Proc Natl Acad Sci U S A, 2018 PubMed PMCID
Value	0.2502
Dimension	pmol/106 cell
Error	Error not provided.
Data presentation in publication	diagram

Source
Source organism	mouse (Mus musculus) Taxonomy 10090
Animal strain	C57BL/6 mice
Sample source	tissue
Tissue type	splenocytes
Cell line/type as in publication	C57BL/6; Samhd1 +/∆25
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	with HIV-1 reverse transcriptase
Growth conditions	splenocytes were activated with 20 μg/mL S. typhosa LPS plus 20 ng/mL mouse IL-4 for 24 h; activated cells were then transduced with pMiG-derived retroviruses that expressed mKO2-cdt; dNTPmeasurement was 2 days after transfection from mKO2+ cells; G1 phase cells

Treatment
Drug/stress type	drug
Applied drug	LPS, IL-4

Genes and Proteins
Gene name	SAMHD1
Gene identifier	56045
Protein name	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein identifier	Q60710
Genetic manipulation/variation	mutation
Mutation in protein	25-bp frame-shift deletions within Exon 1, heterozygous mutation, fusion protein