DNTP003754
dATP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000948 |
| Members of this Pool |
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| Members of control pools | ||
|---|---|---|
| POOL000942 | POOL000943 | POOL000943 |
|
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Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Lahouassa H, Daddacha W, Hofmann H, Ayinde D, Logue EC, Dragin L, Bloch N, Maudet C, Bertrand M, Gramberg T, Pancino G, Priet S, Canard B, Laguette N, Benkirane M, Transy C, Landau NR, Kim B, Margottin-Goguet F: SAMHD1 restricts the replication of human immunodeficiency virus type 1 by depleting the intracellular pool of deoxynucleoside triphosphates.,
Nat Immunol, 2012
PubMed PMCID |
| Value | 29.3548 | Dimension | nM |
| Error (unspecified) | 1.9355 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| CLO Cell line name | U-937 cell (CLO:0009465) |
| Cell line/type as in publication | U937 cells |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | with HIV-1 reverse transcriptase |
| Growth conditions | PMA |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 27924 |
| Applied drug | phorbol 12-myristate 13-acetate |
| Treatment details/effects | human HD206-7AA SAMHD1 expressing vector |
| Genes and Proteins | |
|---|---|
| Gene name | SAMHD1 |
| Gene identifier | 25939 |
| Protein name | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 |
| Protein identifier | Q9Y3Z3 |
| Genetic manipulation/variation | extrachromosomal expression of mutant protein |
| Mutation in protein | consensus histidine and aspartate residues mutated to alanine |