DNTP003683
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000930 |
| Members of this Pool |
|
| Members of control pools |
|---|
| POOL000935 |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Lahouassa H, Daddacha W, Hofmann H, Ayinde D, Logue EC, Dragin L, Bloch N, Maudet C, Bertrand M, Gramberg T, Pancino G, Priet S, Canard B, Laguette N, Benkirane M, Transy C, Landau NR, Kim B, Margottin-Goguet F: SAMHD1 restricts the replication of human immunodeficiency virus type 1 by depleting the intracellular pool of deoxynucleoside triphosphates.,
Nat Immunol, 2012
PubMed PMCID |
| Value | 0.044 | Dimension | pmol/106 cell |
| Error (standard deviation) | 0.002 |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | primary cells |
| Cell line/type as in publication | monocyte-derived macrophages (MDM) |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | with HIV-1 reverse transcriptase |
| Growth conditions | HIV-1 infection; pre-incubated with Vpx containing VLP (virus-like particles); 2 mM hydroxyurea |
| Treatment | |
|---|---|
| Drug/stress type | drug + stress |
| Stress type | viral infection; virus-like particle Vpx treatment |
| PubChem | 3657 |
| Applied drug | hydroxyurea |
| Treatment details/effects | HIV-1 infection; RNR inhibition |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |