Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
POOL000882
Last modified: 10/Sep/2021
Results
Publication
Castillo-Acosta VM, Estévez AM, Vidal AE, Ruiz-Perez LM, González-Pacanowska D: Depletion of dimeric all-alpha dUTPase induces DNA strand breaks and impairs cell cycle progression in Trypanosoma brucei., Int J Biochem Cell Biol, 2008

PubMed
Value 4.1
Dimension pmol/106 cell
Error (unspecified) 0.9
Data presentation in publication numerical
Source
Source organism Trypanosoma brucei brucei
Taxonomy 5702
Sample source strain
Cell line/type as in publication procyclic cell line 449
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Horowitz
Growth conditions depletion of dUTPase activity by RNAi; cells in procyclics grown in the presence of doxycyclin for 8 days
Treatment
Drug/stress type drug
PubChem 54671203
Applied drug doxycyclin
Treatment details/effects depletion of dUTPase activity by RNAi
Genes and Proteins
Gene name Tb927.7.5160
Gene identifier 3658718
Protein name Deoxyuridine triphosphatase, putative
Protein identifier Q57ZH3
Genetic manipulation/variation silencing