2'-Deoxyadenosine 5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL000525
Members of this Pool
Members of control pools
POOL000516 POOL000524
Last modified: 10/Sep/2021
Results
Publication
Earp C, Rowbotham S, Merényi G, Chabes A, Cha RS: S phase block following MEC1ATR inactivation occurs without severe dNTP depletion., Biol Open, 2015

PubMed PMCID
Value 75.3695
Dimension pmol/108 CFU
Error Error not provided.
Data presentation in publication diagram
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain CEY173 derivative of SK1 strain with MATa genotype. MEC1 allele. Transformed with plasmid carrying GIS2.
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Remarks to measurement type Chabes
Growth conditions Strains were grown to mid-log phase at 23°C before being shifted to 30°C. Samples were collected 30 min after the temperature shift.
Treatment
Drug/stress type stress
Stress type temperature stress
Treatment details/effects Temperature treatment at 30°C
Genes and Proteins
Gene name GIS2
Gene identifier 855466
Protein name Zinc finger protein GIS2
Protein identifier P53849
Genetic manipulation/variation extrachromosomal protein expression