Results
Publication	Itsko M, Schaaper RM: dGTP starvation in Escherichia coli provides new insights into the thymineless-death phenomenon., PLoS Genet, 2014 PubMed PMCID
Value	9.4
Dimension	%
Error (standard deviation)	0.8
Relative compared to	ATP
Data presentation in publication	numerical

Source
Source organism	E. coli (Escherichia coli) Taxonomy 562
Bacterial or fungal strain	MG1655
Sample source	strain
Compartment	whole cell

Experiment Details
Measurement type	HPLC-UV
Extraction method	methanol
Growth conditions	dNTP pools in strains grown without hypoxanthine (Hx) at two hours after withdrawal of Hx

Treatment
Drug/stress type	stress
Treatment details/effects	The optA1 allele of dgt is a promoter-up mutation, which increases gene expression by as much as 50-fold. Defection in the gpt gene, which functions in purine salvage by converting guanine into guanosine monophosphate (GMP). Incubations in the purineless medium

Genes and Proteins
Gene name	dgt
Gene identifier	947177
Protein name	Deoxyguanosinetriphosphate triphosphohydrolase
Protein identifier	P15723
Genetic manipulation/variation	mutation
Mutation in protein	opta 1 mutation
Gene name	gpt
Gene identifier	944817
Protein name	Xanthine-guanine phosphoribosyltransferase
Protein identifier	P0A9M5
Genetic manipulation/variation	mutation
Mutation in protein	knock-out