Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL000512
Members of this Pool
Members of control pools
POOL000510
Last modified: 10/Sep/2021
Results
Publication
Itsko M, Schaaper RM: dGTP starvation in Escherichia coli provides new insights into the thymineless-death phenomenon., PLoS Genet, 2014

PubMed PMCID
Value 2.7
Dimension %
Error (standard deviation) 0.3
Relative compared to ATP
Data presentation in publication numerical
Source
Source organism E. coli (Escherichia coli)
Taxonomy 562
Bacterial or fungal strain MG1655
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method methanol
Growth conditions dNTP pools in strains grown without hypoxanthine (Hx) at four hours after withdrawal of Hx
Treatment
Drug/stress type stress
Treatment details/effects Defection in the gpt gene, which functions in purine salvage by converting guanine into guanosine monophosphate (GMP). Incubations in the purineless medium
Genes and Proteins
Gene name gpt
Gene identifier 944817
Protein name Xanthine-guanine phosphoribosyltransferase
Protein identifier P0A9M5
Genetic manipulation/variation mutation
Mutation in protein knock-out