DNTP001970
dTTP
dNTP Pool | |
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Pool identifier | POOL000508 |
Members of this Pool |
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Members of control pools |
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POOL000507 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Itsko M, Schaaper RM: dGTP starvation in Escherichia coli provides new insights into the thymineless-death phenomenon.,
PLoS Genet, 2014
PubMed PMCID |
Value | 4.9 | Dimension | % |
Error (standard deviation) | 0.2 |
Relative compared to | ATP |
Data presentation in publication | numerical |
Source | |
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Source organism |
E. coli
(Escherichia coli)
Taxonomy 562 |
Bacterial or fungal strain | MG1655 |
Sample source | strain |
Compartment | whole cell |
Experiment Details | |
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Measurement type | HPLC-UV |
Extraction method | methanol |
Growth conditions | dNTP pools in strains grown without hypoxanthine (Hx) at two hours after withdrawal of Hx |
Treatment | |
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Drug/stress type | stress |
Treatment details/effects | The optA1 allele of dgt is a promoter-up mutation, which increases gene expression by as much as 50-fold. Incubations in the purineless medium |
Genes and Proteins | |
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Gene name | dgt |
Gene identifier | 947177 |
Protein name | Deoxyguanosinetriphosphate triphosphohydrolase |
Protein identifier | P15723 |
Genetic manipulation/variation | mutation |
Mutation in protein | opta 1 mutation |