DNTP001903
dCTP
dNTP Pool | |
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This entry does not belong to any pool in the database. |
Members of control pools |
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N/A |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Bianchi V, Pontis E, Reichard P: Changes of deoxyribonucleoside triphosphate pools induced by hydroxyurea and their relation to DNA synthesis.,
J Biol Chem, 1986
PubMed |
Value | 106.4516 | Dimension | pmol/106 cell |
Error | Error not provided. |
Data presentation in publication | diagram |
Source | |
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Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
Animal strain | Swill albino mice |
Sample source | cell line |
CLO Cell line name | 3T6-Swiss albino cell (CLO:0001356) |
Cell line/type as in publication | 3T6 |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | sample was extracted with Freon/octylamine before the assay too |
Growth conditions | Eagle's modification of Dulbecco's medium, 37°C; 60-70% of cells should be in S phase before treatment |
Treatment | |
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Drug/stress type | drug |
PubChem | 3657 , 6175 |
Applied drug | hydroxyurea, [5-^3H]cytidine |
Treatment details/effects | [5-^3H]cytidine for 60 min; then 3 mM hydroxyurea (HU); 10 min after HU remained in original media; 30 min after HU |
Genes and Proteins | |
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No genes or proteins manipulated. | |