2'-Deoxycytidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A
Last modified: 10/Sep/2021
Results
Publication
Bianchi V, Pontis E, Reichard P: Changes of deoxyribonucleoside triphosphate pools induced by hydroxyurea and their relation to DNA synthesis., J Biol Chem, 1986

PubMed
Value 119.0323
Dimension pmol/106 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism mouse (Mus musculus)
Taxonomy 10090
Animal strain Swill albino mice
Sample source cell line
CLO Cell line name 3T6-Swiss albino cell (CLO:0001356)
Cell line/type as in publication 3T6
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type sample was extracted with Freon/octylamine before the assay too
Growth conditions Eagle's modification of Dulbecco's medium, 37°C; 60-70% of cells should be in S phase before treatment
Treatment
Drug/stress type drug
PubChem 3657 , 6175
Applied drug hydroxyurea, [5-^3H]cytidine
Treatment details/effects [5-^3H]cytidine for 60 min; then 3 mM hydroxyurea (HU); 10 min after HU remained in original media; 20 min after HU
Genes and Proteins
No genes or proteins manipulated.