DNTP001867
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000482 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Bianchi V, Pontis E, Reichard P: Changes of deoxyribonucleoside triphosphate pools induced by hydroxyurea and their relation to DNA synthesis.,
J Biol Chem, 1986
PubMed |
| Value | 118 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
| Animal strain | Swill albino mice |
| Sample source | cell line |
| CLO Cell line name | 3T6-Swiss albino cell (CLO:0001356) |
| Cell line/type as in publication | 3T6 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | sample was extracted with Freon/octylamine before the assay too |
| Growth conditions | Eagle's modification of Dulbecco's medium, 37°C; 60-70% of cells should be in S phase before treatment |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 3657 , 6175 |
| Applied drug | hydroxyurea, [5-^3H]cytidine |
| Treatment details/effects | 0.3 μM [5-^3H]cytidine for 60 min; then 3 mM hydroxyurea; 0 min after HU |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |