DNTP001857
dGTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000479 |
| Members of this Pool |
|
| Members of control pools |
|---|
| POOL000474 |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Kumar D, Viberg J, Nilsson AK, Chabes A: Highly mutagenic and severely imbalanced dNTP pools can escape detection by the S-phase checkpoint.,
Nucleic Acids Res, 2010
PubMed PMCID |
| Value | 703.5176 | Dimension | pmol/108 CFU |
| Error (SD) | 25.1256 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
baker's yeast
(Saccharomyces cerevisiae)
Taxonomy 4932 |
| Sample source | strain |
| Cell line/type as in publication | prnr1-Q288E |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | HPLC-UV |
| Extraction method | acid extraction |
| Growth conditions | grown in synthetic complete media without uracil (SC-URA) with 2% galactose (SC-URA+GAL) for at least six generations |
| Treatment | |
|---|---|
| Treatment details/effects | overexpression of mutant Rnr1 |
| Genes and Proteins | |
|---|---|
| Gene name | RNR1 |
| Gene identifier | 856801 |
| Protein name | ribonucleoside-diphosphate reductase large chain 1 |
| Protein identifier | P21524 |
| Genetic manipulation/variation | extrachromosomal expression of mutant protein |
| Mutation in protein | Q288E |