DNTP001627
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000404 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Chimploy K, Tassotto ML, Mathews CK: Ribonucleotide reductase, a possible agent in deoxyribonucleotide pool asymmetries induced by hypoxia.,
J Biol Chem, 2000
PubMed |
| Value | 2.3 | Dimension | fold change |
| Error | Error not provided. |
| Relative compared to | 2% O2 / 20% O2 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
Chinese hamster
(Cricetulus griseus)
Taxonomy 10029 |
| Sample source | cell line |
| Cell line/type as in publication | V79 hamster lung cell cultures |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | radioactive type, North et al. (1980); combined methanol and perchloric acid extraction |
| Growth conditions | 6 minutes result; cells were synchronized by a double 3 μM aphidicolin treatment to bring most of them into S phase at the time the measurement were done |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 457964 |
| Applied drug | Aphidicolin |
| Treatment details/effects | 3 μM aphidicolin treatment prevents progression of cells from G1 into S phase |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |