2-Hydroxymethyl-dCTP (Pubchem)
dNTP Pool
Pool identifier POOL000399
Members of this Pool
Members of control pools
POOL000397
Last modified: 10/Sep/2021
Results
Publication
Ji JP, Mathews CK: Analysis of mutagenesis induced by a thermolabile T4 phage deoxycytidylate hydroxymethylase suggests localized deoxyribonucleotide pool imbalance., Mol Gen Genet, 1991

PubMed
Value 64000
Dimension molecules/cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism E. coli (Escherichia coli)
Taxonomy 562
Bacterial or fungal strain B strain
Sample source strain
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type radioactive type, North et al. (1980); combined methanol and perchloric acid extraction
Growth conditions E. coli B maintained at 34 °C were infected with ts LBS3 at 34 °C, cells were harvested after 20 minutes of infection
Treatment
Drug/stress type stress
Stress type phage infection; temperature stress
Treatment details/effects ts LB3 infection; 34 °C temperature shift
Genes and Proteins
Gene name 42
Gene identifier 1258746
Protein name Deoxycytidylate 5-hydroxymethyltransferase
Protein identifier P08773
Genetic manipulation/variation mutation in infecting virus
Mutation in protein termperature-sensitive mutant