DNTP001560
dTTP
dNTP Pool | |
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Pool identifier | POOL000388 |
Members of this Pool |
|
Members of control pools |
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False |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Ji JP, Mathews CK: Analysis of mutagenesis induced by a thermolabile T4 phage deoxycytidylate hydroxymethylase suggests localized deoxyribonucleotide pool imbalance.,
Mol Gen Genet, 1991
PubMed |
Value | 700000 | Dimension | molecules/cell |
Error | Error not provided. |
Data presentation in publication | diagram |
Source | |
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Source organism |
E. coli
(Escherichia coli)
Taxonomy 562 |
Bacterial or fungal strain | B strain |
Sample source | strain |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | radioactive type, North et al. (1980); combined methanol and perchloric acid extraction |
Growth conditions | infection of cell with ts LB3 at 28 °C for 20 min, then temperature was shifted to 43 °C for 10 min |
Treatment | |
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Drug/stress type | stress |
Stress type | phage infection; temperature stress |
Treatment details/effects | ts LB3 infection; 43 °C temperature shift |
Genes and Proteins | |
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Gene name | 42 |
Gene identifier | 1258746 |
Protein name | Deoxycytidylate 5-hydroxymethyltransferase |
Protein identifier | P08773 |
Genetic manipulation/variation | mutation in infecting virus |
Mutation in protein | termperature-sensitive mutant |