DNTP001469
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000365 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Mun BJ, Mathews CK: Cell cycle-dependent variations in deoxyribonucleotide metabolism among Chinese hamster cell lines bearing the Thy- mutator phenotype.,
Mol Cell Biol, 1991
PubMed PMCID |
| Value | 27 | Dimension | pmol/106 nuclei |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
Chinese hamster
(Cricetulus griseus)
Taxonomy 10029 |
| Sample source | cell line |
| CLO Cell line name | CHO K1 cell (CLO:0002427) |
| Cell line/type as in publication | CHO-K1 |
| Compartment | nucleus |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | radioactive type, North et al. (1980); combined methanol and perchloric acid extraction |
| Growth conditions | Synchronized, S-phase-enriched cells cells. Cultures in early exponential growth were transferred to medium containing dialyzed serum and 100 μM thymidine. After 2 days of further growth, cells were synchronized by 30 h of isoleucine deprivation, also in medium containing 100 μM thymidine. At 12 h after isoleucine readdition, dNTP pools were extracted from rapidly isolated nuclei. |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 5789 |
| Applied drug | Thymidine |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |