DNTP001394
dCTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000346 |
| Members of this Pool |
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Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Mun BJ, Mathews CK: Cell cycle-dependent variations in deoxyribonucleotide metabolism among Chinese hamster cell lines bearing the Thy- mutator phenotype.,
Mol Cell Biol, 1991
PubMed PMCID |
| Value | 3 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
Chinese hamster
(Cricetulus griseus)
Taxonomy 10029 |
| Sample source | cell line |
| CLO Cell line name | CHO K1 cell (CLO:0002427) |
| Cell line/type as in publication | CHO-K1 |
| Compartment | nucleus |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | radioactive type, North et al. (1980); combined methanol and perchloric acid extraction |
| Growth conditions | Exponentially growing wild-type CHO-K1 cells in 5 μM thymidine were treated with NP-40-containing buffer for 120 seconds, and the nuclear dNTPs were extracted, quantitated, and compared with corresponding values for a whole-cell (WC) extract |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 5789 |
| Applied drug | Thymidine |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |