DNTP001328
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000330 |
| Members of this Pool |
|
| Members of control pools |
|---|
| POOL000327 |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Pajalunga D, Franzolin E, Stevanoni M, Zribi S, Passaro N, Gurtner A, Donsante S, Loffredo D, Losanno L, Bianchi V, Russo A, Rampazzo C, Crescenzi M: A defective dNTP pool hinders DNA replication in cell cycle-reactivated terminally differentiated muscle cells.,
Cell Death Differ, 2017
PubMed PMCID |
| Value | 27 | Dimension | pmol/A260nm |
| Error (standard error of the mean) | 0 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
| Sample source | primary cells |
| Tissue type | muscle |
| Cell line/type as in publication | myotube |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Growth conditions | Cells were collected by centrifugation and plated on gelatin-coated dishes in growth medium (GM): Ham’s F-10 medium supplemented with 20% fetal bovine serum (FBS), 3% chicken embryo extract, and 2.5 ng/ml basic-FGF. Infected with adenovirus E1A protein Ad-E1A. 48 hr after infection, the cells were harvested. |
| Treatment | |
|---|---|
| Treatment details/effects | Cells were infected with E1A expressing dl520 mutant adenovirus; E1A has the ability to activate DNA synthesis in reversibly growth-arrested cells |
| Genes and Proteins | |
|---|---|
| Gene name | not available |
| Gene identifier | Not available. |
| Protein name | Early E1A protein |
| Protein identifier | P03255 |
| Genetic manipulation/variation | extrachromosomal protein expression |