DNTP001294
dCTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000321 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Pajalunga D, Franzolin E, Stevanoni M, Zribi S, Passaro N, Gurtner A, Donsante S, Loffredo D, Losanno L, Bianchi V, Russo A, Rampazzo C, Crescenzi M: A defective dNTP pool hinders DNA replication in cell cycle-reactivated terminally differentiated muscle cells.,
Cell Death Differ, 2017
PubMed PMCID |
| Value | 34.4 | Dimension | pmol/A260nm |
| Error | Error not provided. |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
| Sample source | primary cells |
| Tissue type | muscle |
| Cell line/type as in publication | formerly quiescent myoblasts reactivated by replating |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Growth conditions | Cells were collected by centrifugation and plated on gelatin-coated dishes in growth medium (GM): Ham’s F-10 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 20% fetal bovine serum (FBS), 3% chicken embryo extract, and 2.5 ng/ml basic-FGF.Spontaneous immortalization occurred within 10 passages. Differentiation was induced by plating cells on gelatin-coated dishes in differentiation medium: Dulbecco’s Modified Eagle Medium (Life Technologies) supplemented with 10% FBS. After 48 h, the vast majority of the cells were differentiated (differentiation index >95%). |
| Treatment | |
|---|---|
| Treatment details/effects | Cells were harvested after 20 hours |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |