Results
Publication	Frangini M, Franzolin E, Chemello F, Laveder P, Romualdi C, Bianchi V, Rampazzo C: Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes., J Biol Chem, 2013 PubMed PMCID
Value	18.3
Dimension	pmol/A260nm
Error (standard deviation)	1.3
Data presentation in publication	diagram

Source
Source organism	mouse (Mus musculus) Taxonomy 10090
Sample source	cell line
Tissue type	muscle
Cell line/type as in publication	isolated myotube
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Growth conditions	grown at 37 °C in a controlled humidified 5% CO2 atmosphere. They were maintained in gelatin-coated plates in proliferation medium (PM) (Dulbecco's modified Eagle's medium supplemented with 10% FCS) up to 60% confluence. To initiate differentiation, cells were allowed to reach 90% confluence, and the medium was changed to differentiation medium (DM, the same as PM but with 2% horse serum). Myotubes were separated from the mononucleated cells present in fully differentiated cultures (DC) on the basis of their different adhesion properties.

Treatment
Drug/stress type	drug
Applied drug	CdR
Treatment details/effects	Purified myotubes transfected for 4 days in DM with anti TK2 siRNAs and incubated with 500 μM CdR for the last 24 h

Genes and Proteins
Gene name	Tk2
Gene identifier	57813
Protein name	Thymidine kinase 2, mitochondrial
Protein identifier	Q8BN51
Genetic manipulation/variation	silencing