DNTP001184
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000294 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Frangini M, Franzolin E, Chemello F, Laveder P, Romualdi C, Bianchi V, Rampazzo C: Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes.,
J Biol Chem, 2013
PubMed PMCID |
| Value | 6.8 | Dimension | pmol/A260nm |
| Error (standard deviation) | 1.6 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
| Sample source | cell line |
| Tissue type | muscle |
| Cell line/type as in publication | isolated myotube |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Growth conditions | grown at 37 °C in a controlled humidified 5% CO2 atmosphere. They were maintained in gelatin-coated plates in proliferation medium (PM) (Dulbecco's modified Eagle's medium supplemented with 10% FCS) up to 60% confluence. To initiate differentiation, cells were allowed to reach 90% confluence, and the medium was changed to differentiation medium (DM, the same as PM but with 2% horse serum). Myotubes were separated from the mononucleated cells present in fully differentiated cultures (DC) on the basis of their different adhesion properties. |
| Treatment | |
|---|---|
| Drug/stress type | stress |
| Treatment details/effects | Purified myotubes transfected for 4 days in DM with control siRNA |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |