Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
POOL000286
Last modified: 10/Sep/2021
Results
Publication
Frangini M, Franzolin E, Chemello F, Laveder P, Romualdi C, Bianchi V, Rampazzo C: Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes., J Biol Chem, 2013

PubMed PMCID
Value 4.6
Dimension pmol/A260nm
Error (standard deviation) 0.9
Data presentation in publication diagram
Source
Source organism mouse (Mus musculus)
Taxonomy 10090
Sample source cell line
Tissue type muscle
Cell line/type as in publication differentiated culture
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Growth conditions grown at 37 °C in a controlled humidified 5% CO2 atmosphere. They were maintained in gelatin-coated plates in proliferation medium (PM) (Dulbecco's modified Eagle's medium supplemented with 10% FCS) up to 60% confluence. To initiate differentiation, cells were allowed to reach 90% confluence, and the medium was changed to differentiation medium (DM, the same as PM but with 2% horse serum).
Treatment
Drug/stress type drug
Applied drug [3H]CdR
Treatment details/effects 0.025 μM [3H]CdR (3H-labelled thymidine)
Genes and Proteins
No genes or proteins manipulated.