DNTP001178
dTTP
dNTP Pool | |
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This entry does not belong to any pool in the database. |
Members of control pools |
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POOL000286 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Frangini M, Franzolin E, Chemello F, Laveder P, Romualdi C, Bianchi V, Rampazzo C: Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes.,
J Biol Chem, 2013
PubMed PMCID |
Value | 4.6 | Dimension | pmol/A260nm |
Error (standard deviation) | 0.9 |
Data presentation in publication | diagram |
Source | |
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Source organism |
mouse
(Mus musculus)
Taxonomy 10090 |
Sample source | cell line |
Tissue type | muscle |
Cell line/type as in publication | differentiated culture |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Growth conditions | grown at 37 °C in a controlled humidified 5% CO2 atmosphere. They were maintained in gelatin-coated plates in proliferation medium (PM) (Dulbecco's modified Eagle's medium supplemented with 10% FCS) up to 60% confluence. To initiate differentiation, cells were allowed to reach 90% confluence, and the medium was changed to differentiation medium (DM, the same as PM but with 2% horse serum). |
Treatment | |
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Drug/stress type | drug |
Applied drug | [3H]CdR |
Treatment details/effects | 0.025 μM [3H]CdR (3H-labelled thymidine) |
Genes and Proteins | |
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No genes or proteins manipulated. | |