DNTP001134
dTTP
| dNTP Pool | |
|---|---|
| This entry does not belong to any pool in the database. |
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Bianchi V, Borella S, Rampazzo C, Ferraro P, Calderazzo F, Bianchi LC, Skog S, Reichard P: Cell cycle-dependent metabolism of pyrimidine deoxynucleoside triphosphates in CEM cells.,
J Biol Chem, 1997
PubMed |
| Value | 30.8 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| CLO Cell line name | CCRF-CEM cell (CLO:0002334) |
| Cell line/type as in publication | CEM CCL 119 neoplastic T lymphocyte |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Growth conditions | Dulbecco's modified Eagle's medium (DMEM) supplemented with 7.5% fetal calf serum, glutamine, and antibiotics, at 37 °C and 5% CO2 in a humidified incubator |
| Treatment | |
|---|---|
| Treatment details/effects | Fraction 5 (cells were separated by elutriation after 60 min of incubation with tritiated thymidine) |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |