DNTP001124
dGTP
dNTP Pool | |
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Pool identifier | POOL000279 |
Members of this Pool |
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Members of control pools |
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POOL000277 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Buckland RJ, Watt DL, Chittoor B, Nilsson AK, Kunkel TA, Chabes A: Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.,
PLoS Genet, 2014
PubMed PMCID |
Value | 46.15 | Dimension | pmol/108 cell |
Error (standard error of the mean) | 3.85 |
Data presentation in publication | diagram |
Source | |
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Source organism |
baker's yeast
(Saccharomyces cerevisiae)
Taxonomy 4932 |
Bacterial or fungal strain | rnr1-Y285A msh2∆ (AC402 derivative) |
Sample source | strain |
Compartment | whole cell |
Experiment Details | |
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Measurement type | HPLC-UV |
Extraction method | acid extraction |
Growth conditions | YPAD, 30 °C |
Treatment | |
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Treatment details/effects | deletion of DNA mismatch repair protein MSH2, component of the post-replicative DNA mismatch repair system (MMR); mutation in ribonucleotide reductase responsible for deoxynucleotide synthesis |
Genes and Proteins | |
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Gene name | RNR1 |
Gene identifier | 856801 |
Protein name | Ribonucleoside-diphosphate reductase large chain 1 |
Protein identifier | P21524 |
Genetic manipulation/variation | mutation |
Mutation in protein | Y285A |
Gene name | MSH2 |
Gene identifier | 854063 |
Protein name | DNA mismatch repair protein MSH2 |
Protein identifier | P25847 |
Genetic manipulation/variation | mutation |
Mutation in protein | deletion |