Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL000264
Members of this Pool
Members of control pools
POOL000259
Last modified: 10/Sep/2021
Results
Publication
Hoch NC, Chen ES, Buckland R, Wang SC, Fazio A, Hammet A, Pellicioli A, Chabes A, Tsai MD, Heierhorst J: Molecular basis of the essential s phase function of the rad53 checkpoint kinase., Mol Cell Biol, 2013

PubMed PMCID
Value 27.92
Dimension pmol/108 cell
Error (standard error of the mean) 0.48
Data presentation in publication diagram
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain T15-3AQ (W303-1A derivative)
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Growth conditions asynchronous cultures grown on YPD
Treatment
Treatment details/effects mutations in Serine/threonine-protein kinase RAD53, involved in S-phase checkpoint
Genes and Proteins
Gene name RAD53
Gene identifier 855950
Protein name Serine/threonine-protein kinase RAD53
Protein identifier P22216
Genetic manipulation/variation mutation
Mutation in protein T15-3AQ mutation; preserving a single threonine (T15) in the N-terminal phosphorylation cluster