Results
Publication	Hoch NC, Chen ES, Buckland R, Wang SC, Fazio A, Hammet A, Pellicioli A, Chabes A, Tsai MD, Heierhorst J: Molecular basis of the essential s phase function of the rad53 checkpoint kinase., Mol Cell Biol, 2013 PubMed PMCID
Value	7.96
Dimension	pmol/108 cell
Error (standard error of the mean)	1.03
Data presentation in publication	diagram

Source
Source organism	baker's yeast (Saccharomyces cerevisiae) Taxonomy 4932
Bacterial or fungal strain	T5-3AQ (W303-1A derivative)
Sample source	strain
Compartment	whole cell

Experiment Details
Measurement type	HPLC-UV
Extraction method	acid extraction
Growth conditions	asynchronous cultures grown on YPD

Treatment
Treatment details/effects	mutations in Serine/threonine-protein kinase RAD53, involved in S-phase checkpoint

Genes and Proteins
Gene name	RAD53
Gene identifier	855950
Protein name	Serine/threonine-protein kinase RAD53
Protein identifier	P22216
Genetic manipulation/variation	mutation
Mutation in protein	T5-3AQ mutation; preserving a single threonine (T5) in the N-terminal phosphorylation cluster