Results
Publication	Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P: dNTP pools determine fork progression and origin usage under replication stress., EMBO J, 2012 PubMed PMCID
Value	685.39
Dimension	pmol/108 cell
Error	Error not provided.
Data presentation in publication	diagram

Source
Source organism	baker's yeast (Saccharomyces cerevisiae) Taxonomy 4932
Bacterial or fungal strain	mec1-100 sml1∆ (W303 derivative)
Sample source	strain
Compartment	whole cell

Experiment Details
Measurement type	HPLC-UV
Extraction method	acid extraction
Growth conditions	YEP medium supplemented with glucose or galactose; cells were synchronized in G1 by adding α-factor for 170 min ; then cells were released into YPD with 200 mM hydroxyurea for 60 min

Treatment
Drug/stress type	drug
PubChem	22461951 , 3657
Applied drug	α-factor, hydroxyurea
Treatment details/effects	synchronization of cells in G1, then cells were treated with hydroxyurea, which inhibits deoxynuclotide synthesis; mutation(s) in Serine/threonine-protein kinase MEC1 and Ribonucleotide reductase inhibitor protein SML1, involved in S-phase checkpoint

Genes and Proteins
Gene name	MEC1
Gene identifier	852433
Protein name	Serine/threonine-protein kinase MEC1
Protein identifier	P38111
Genetic manipulation/variation	mutation
Mutation in protein	mec1-100 mutation impairs both the G1/S and intra-S damage checkpoints
Gene name	SML1
Gene identifier	854945
Protein name	Ribonucleotide reductase inhibitor protein SML1
Protein identifier	Q04964
Genetic manipulation/variation	mutation
Mutation in protein	deletion