Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL000242
Members of this Pool
Members of control pools
POOL000231
Last modified: 10/Sep/2021
Results
Publication
Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P: dNTP pools determine fork progression and origin usage under replication stress., EMBO J, 2012

PubMed PMCID
Value 1292.13
Dimension pmol/108 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain ctf4∆ (W303 derivative)
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Growth conditions YEP medium supplemented with glucose or galactose; cells were synchronized in G1 by adding α-factor for 170 min ; then cells were released into YPD with 200 mM hydroxyurea for 60 min
Treatment
Drug/stress type drug
PubChem 22461951 , 3657
Applied drug α-factor, hydroxyurea
Treatment details/effects synchronization of cells in G1, then cells were treated with hydroxyurea, which inhibits deoxynuclotide synthesis; mutation(s) in DNA polymerase alpha-binding protein, involved in maintaining replication fork integrity
Genes and Proteins
Gene name CTF4
Gene identifier 856254
Protein name DNA polymerase alpha-binding protein
Protein identifier Q01454
Genetic manipulation/variation mutation
Mutation in protein deletion