DNTP000950
dTTP
dNTP Pool | |
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Pool identifier | POOL000237 |
Members of this Pool |
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Members of control pools |
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POOL000231 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P: dNTP pools determine fork progression and origin usage under replication stress.,
EMBO J, 2012
PubMed PMCID |
Value | 1127.34 | Dimension | pmol/108 cell |
Error | Error not provided. |
Data presentation in publication | diagram |
Source | |
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Source organism |
baker's yeast
(Saccharomyces cerevisiae)
Taxonomy 4932 |
Bacterial or fungal strain | rad52∆ (W303 derivative) |
Sample source | strain |
Compartment | whole cell |
Experiment Details | |
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Measurement type | HPLC-UV |
Extraction method | acid extraction |
Growth conditions | YEP medium supplemented with glucose or galactose; cells were synchronized in G1 by adding α-factor for 170 min ; then cells were released into YPD with 200 mM hydroxyurea for 60 min |
Treatment | |
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Drug/stress type | drug |
PubChem | 22461951 , 3657 |
Applied drug | α-factor, hydroxyurea |
Treatment details/effects | synchronization of cells in G1, then cells were treated with hydroxyurea, which inhibits deoxynuclotide synthesis; mutation(s) in DNA repair and recombination protein RAD52, involved in homologous recombination |
Genes and Proteins | |
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Gene name | RAD52 |
Gene identifier | 854976 |
Protein name | DNA repair and recombination protein RAD52 |
Protein identifier | P06778 |
Genetic manipulation/variation | mutation |
Mutation in protein | deletion |