Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL000237
Members of this Pool
Members of control pools
POOL000231
Last modified: 10/Sep/2021
Results
Publication
Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P: dNTP pools determine fork progression and origin usage under replication stress., EMBO J, 2012

PubMed PMCID
Value 1127.34
Dimension pmol/108 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain rad52∆ (W303 derivative)
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Growth conditions YEP medium supplemented with glucose or galactose; cells were synchronized in G1 by adding α-factor for 170 min ; then cells were released into YPD with 200 mM hydroxyurea for 60 min
Treatment
Drug/stress type drug
PubChem 22461951 , 3657
Applied drug α-factor, hydroxyurea
Treatment details/effects synchronization of cells in G1, then cells were treated with hydroxyurea, which inhibits deoxynuclotide synthesis; mutation(s) in DNA repair and recombination protein RAD52, involved in homologous recombination
Genes and Proteins
Gene name RAD52
Gene identifier 854976
Protein name DNA repair and recombination protein RAD52
Protein identifier P06778
Genetic manipulation/variation mutation
Mutation in protein deletion