DNTP000925
dCTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000231 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P: dNTP pools determine fork progression and origin usage under replication stress.,
EMBO J, 2012
PubMed PMCID |
| Value | 45.03 | Dimension | pmol/108 cell |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
baker's yeast
(Saccharomyces cerevisiae)
Taxonomy 4932 |
| Bacterial or fungal strain | wt (W303) |
| Sample source | strain |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | HPLC-UV |
| Extraction method | acid extraction |
| Growth conditions | YEP medium supplemented with glucose or galactose; cells were synchronized in G1 by adding α-factor for 170 min ; then cells were released into YPD with 200 mM hydroxyurea for 60 min |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 22461951 , 3657 |
| Applied drug | α-factor, hydroxyurea |
| Treatment details/effects | synchronization of cells in G1, then cells were treated with hydroxyurea, which inhibits deoxynuclotide synthesis |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |