Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL000227
Members of this Pool
Members of control pools
POOL000217
Last modified: 10/Sep/2021
Results
Publication
Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P: dNTP pools determine fork progression and origin usage under replication stress., EMBO J, 2012

PubMed PMCID
Value 1212.01
Dimension pmol/108 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain rrm3∆ (W303 derivative)
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Growth conditions YEP medium supplemented with glucose or galactose; cells were synchronized in G1 by adding α-factor for 170 min
Treatment
Drug/stress type drug
PubChem 22461951
Applied drug α-factor
Treatment details/effects synchronization of cells in G1; mutation(s) in ATP-dependent DNA helicase RRM3, involved in maintaining replication fork integrity
Genes and Proteins
Gene name RRM3
Gene identifier 856426
Protein name ATP-dependent DNA helicase RRM3
Protein identifier P38766
Genetic manipulation/variation mutation
Mutation in protein deletion