Thymidine-5'-triphosphate (Pubchem)
Deoxyuridine triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL000155
Members of this Pool
Members of control pools
POOL000149
Last modified: 10/Sep/2021
Results
Publication
Wanrooij PH, Engqvist MKM, Forslund JME, Navarrete C, Nilsson AK, Sedman J, Wanrooij S, Clausen AR, Chabes A: Ribonucleotides incorporated by the yeast mitochondrial DNA polymerase are not repaired., Proc Natl Acad Sci U S A, 2017

PubMed PMCID
Value 3.4592
Dimension pmol/105 cells
Error (standard error of the mean) 0.2972
Data presentation in publication diagram
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain strains congenic to W1588-4C
Sample source strain
Cell line/type as in publication RNH201−
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Remarks to measurement type Chabes (2015)
Growth conditions N/A
Treatment
Treatment details/effects rnh201∆ strain, deficient for ribonucleotide excision repair (RER); mutation in ribonucleotide reductase responsible for deoxynucleotide synthesis
Genes and Proteins
Gene name rnr1
Gene identifier 856801
Protein name Ribonucleoside-diphosphate reductase large chain 1
Protein identifier P21524
Genetic manipulation/variation mutation
Mutation in protein Y285A, Q288E