|Members of this Pool
Last modified: 10/Sep/2021
Wojcik BE, Dermody JJ, Ozer HL, Mun B, Mathews CK: Temperature-sensitive DNA mutant of Chinese hamster ovary cells with a thermolabile ribonucleotide reductase activity., Mol Cell Biol, 1990
|Error not provided.
|Data presentation in publication
|Cell line/type as in publication
|3B2R1 (JB3-B revertant cell line)
|dNTP incorporation assay
|Remarks to measurement type
|Two-step extraction by North et al modified: 5% trichloroacetic acid was used in place of 5% perchloric acid, acid extracts were neutralized with tri-N-octylamine as in Garret and Santi.
|Cell line in which the unknown temperature sensitive mutation of JB3-B is reverted back. Shows no DNA replication defect upon thermal shift from 33 C to 39 C. Cultured similarly to JB3-B.
|There was no treatment in this experiment.
|Genes and Proteins
|No genes or proteins manipulated.