Results
Publication	Wojcik BE, Dermody JJ, Ozer HL, Mun B, Mathews CK: Temperature-sensitive DNA mutant of Chinese hamster ovary cells with a thermolabile ribonucleotide reductase activity., Mol Cell Biol, 1990 PubMed PMCID
Value	5
Dimension	pmol/106 cell
Error	Error not provided.
Data presentation in publication	diagram

Source
Source organism	Chinese hamster (Cricetulus griseus) Taxonomy 10029
Sample source	cell line
Cell line/type as in publication	JB3-B (CHO-S derived mutant for DNA replication)
Compartment	nucleus

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Two-step extraction by North et al modified: 5% trichloroacetic acid was used in place of 5% perchloric acid, acid extracts were neutralized with tri-N-octylamine as in Garret and Santi.
Growth conditions	Cultured in a 1:1 mixture of DMEM and F12 Ham's medium (DF medium) supplemented with 10% newborn or fetal bovine serum. Cell line is temperature sensitive for DNA replication. Shift from 33 C to 39 C inhibits DNA synthesis.

Treatment
Drug/stress type	stress
Stress type	temperature stress
Treatment details/effects	t = 3 h at 39 C

Genes and Proteins
No genes or proteins manipulated.