DNTP000195
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000049 |
| Members of this Pool |
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Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Wojcik BE, Dermody JJ, Ozer HL, Mun B, Mathews CK: Temperature-sensitive DNA mutant of Chinese hamster ovary cells with a thermolabile ribonucleotide reductase activity.,
Mol Cell Biol, 1990
PubMed PMCID |
| Value | 152 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
Chinese hamster
(Cricetulus griseus)
Taxonomy 10029 |
| Sample source | cell line |
| Cell line/type as in publication | JB3-B (CHO-S derived mutant for DNA replication) |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | Two-step extraction by North et al modified: 5% trichloroacetic acid was used in place of 5% perchloric acid, acid extracts were neutralized with tri-N-octylamine as in Garret and Santi. |
| Growth conditions | Cultured in a 1:1 mixture of DMEM and F12 Ham's medium (DF medium) supplemented with 10% newborn or fetal bovine serum. Cell line is temperature sensitive for DNA replication. Shift from 33 C to 39 C inhibits DNA synthesis. Control is measured from cells kept at 33 C. |
| Treatment | |
|---|---|
| There was no treatment in this experiment. |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |