DNTP000177
dCTP
dNTP Pool | |
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Pool identifier | POOL000044 |
Members of this Pool |
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Members of control pools |
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POOL000041 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Arecco A, Mun BJ, Mathews CK: Deoxyribonucleotide pools as targets for mutagenesis by N-methyl-N-nitrosourea.,
Mutat Res, 1988
PubMed |
Value | 45 | Dimension | pmol/106 cell |
Error | Error not provided. |
Data presentation in publication | numerical |
Source | |
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Source organism |
Chinese hamster
(Cricetulus griseus)
Taxonomy 10029 |
Sample source | cell line |
Cell line/type as in publication | V79 hamster lung fibroblasts |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | Two-step extraction using 60 % aqueous methanol followed by 0.5 N perchloric acid. |
Growth conditions | Cells were grown as attached cultures in Dulbecco's Modified Eagle Medium containing 5 % fetal calf serum. |
Treatment | |
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Drug/stress type | drug |
PubChem | 135398592 , 135518164 |
Applied drug | deoxyguanosine, 8-aminoguanosine |
Treatment details/effects | Sub-confluent cultures were incubated for 30 min with 0.1 mM of aminoguanosine and 0.2 mM 8-aminoguanosine |
Genes and Proteins | |
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No genes or proteins manipulated. | |