Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Last modified: 10/Sep/2021
Results
Publication
Mathews CK, Sinha NK: Are DNA precursors concentrated at replication sites?, Proc Natl Acad Sci U S A, 1982

PubMed PMCID
Value 65
Dimension μM
Error Error not provided.
Data presentation in publication numerical
Source
Source organism E. coli (Escherichia coli)
Taxonomy 562
Bacterial or fungal strain Escherichia coli 201
Sample source strain
Compartment whole cell
Experiment Details
Measurement type 2D thin-layer chromatography
Extraction method methanol
Remarks to measurement type 1 % toluene in 60 % aqueous methanol, radioactive detection
Growth conditions Search for minimal dTTP concentration for maximal DNA synthesis rate
Treatment
Drug/stress type stress
Stress type phage infection
Treatment details/effects Infection with td8nd28 T4 phage. Value corresponds to infections of wild-type bacteria by wild-type phage.This value was found saturating for DNA replication.
Genes and Proteins
No genes or proteins manipulated.