DNTP000161
dTTP
dNTP Pool | |
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This entry does not belong to any pool in the database. |
Last modified: 10/Sep/2021
Results | |
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Publication |
Mathews CK, Sinha NK: Are DNA precursors concentrated at replication sites?,
Proc Natl Acad Sci U S A, 1982
PubMed PMCID |
Value | 65 | Dimension | μM |
Error | Error not provided. |
Data presentation in publication | numerical |
Source | |
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Source organism |
E. coli
(Escherichia coli)
Taxonomy 562 |
Bacterial or fungal strain | Escherichia coli 201 |
Sample source | strain |
Compartment | whole cell |
Experiment Details | |
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Measurement type | 2D thin-layer chromatography |
Extraction method | methanol |
Remarks to measurement type | 1 % toluene in 60 % aqueous methanol, radioactive detection |
Growth conditions | Search for minimal dTTP concentration for maximal DNA synthesis rate |
Treatment | |
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Drug/stress type | stress |
Stress type | phage infection |
Treatment details/effects | Infection with td8nd28 T4 phage. Value corresponds to infections of wild-type bacteria by wild-type phage.This value was found saturating for DNA replication. |
Genes and Proteins | |
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No genes or proteins manipulated. | |