DNTP000013
dTTP + dUTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL000004 |
| Members of this Pool |
|
| Members of control pools |
|---|
| POOL000001 |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Cserepes M, Türk D, Tóth S, Pape VFS, Gaál A, Gera M, Szabó JE, Kucsma N, Várady G, Vértessy BG, Streli C, Szabó PT, Tovari J, Szoboszlai N, Szakács G: Unshielding Multidrug Resistant Cancer through Selective Iron Depletion of P-Glycoprotein-Expressing Cells.,
Cancer Res, 2020
PubMed |
| Value | 27.6 | Dimension | pmol/106 cell |
| Error (standard error of the mean) | 4.7 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| CLO Cell line name | MES-SA cell (CLO:0007677) |
| Cell line/type as in publication | MES-SA/B1 (derived from MES-SA, ABCB1 overexpression) |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Wilson, Szabo et al. |
| Growth conditions | Cultured cells were treated with DMSO and with the indicated drug for 24 hours. |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 326326 |
| Applied drug | NSC297366 |
| Treatment details/effects | iron depletion; perturbation in deoxynucleotide metabolism; increased expression of ABCB1 transporter |
| Genes and Proteins | |
|---|---|
| Gene name | ABCB1 |
| Gene identifier | 5243 |
| Protein name | ATP-dependent translocase ABCB1 |
| Protein identifier | P08183 |
| Genetic manipulation/variation | extrachromosomal protein expression |